inverted microscope model eclipse te-2000 u  (Nikon)

Journal: Molecular Vision

Article Title: RPE and neuronal differentiation of allotransplantated porcine ciliary epithelium-derived cells

doi:

Figure Lengend Snippet: Microphotographs of the immunolabeling of newborn pig ciliary epithelium (CE)-derived cells after in vitro differentiation on poly-D-Lysine, laminin coated coverslips in the presence of 1% serum and 10 ng/ml basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). The images were acquired using an epifluorescent microscope. A , B : Cells labeled for recoverin are clustered together (arrows). C : 4',6-diamidino-2-phenylindole (DAPI) nuclear staining corresponding to A and B . D , E : Cells double-labeled for recoverin (red, D ) and rhodopsin (green, D and E ) are depicted by arrows. The focus is set to show rhodopsin-positive cell processes. Strong recoverin staining in the cytoplasm masks the nuclear DAPI staining, which is shown separately in F . Cells positive for protein kinase α (PKCα; green in G and H , arrows) did not co-label with recoverin (red in G and H and another example in the inset in G , arrowheads). The focus is set to show the processes of PKCα-labeled cells in G and H , and the recoverin-labeled processes in the inset in G . Corresponding DAPI nuclear stain is shown in I . J - K : A calbindin immunopositive cell is depicted by the arrow. The focus is set to show the processes of the labeled cell. Corresponding DAPI nuclear stain is shown in L . M , N : Recoverin-positive cells (arrows in M and N ) that had retained pigmented granules (arrow in the bright-field image in O ). P , Q : RPE-65 immunopositive cells (arrows) and the corresponding nuclear DAPI staining in R .

Article Snippet: Immunoreactive cells were visualized and images were recorded using an inverted confocal microscope (Nikon, Model Eclipse TE 2000-U, Tokyo, Japan) and Nikon EZ-C1 software.

Techniques: Immunolabeling, Derivative Assay, In Vitro, Microscopy, Labeling, Staining